Аннотация:
The novel C5-modified nucleotides containing indodicarbocyanine dye bearing a carboxyl group at the 3-position of indolenine have been synthesized and incorporated into DNA by commercially available thermostable Taq DNA polymerase. The efficiency of incorporation of labeled nucleotides during polymerase chain reaction was studied in a TB-biochip and THROMBO-Biochip test systems.
Образец цитирования:
V. E. Kuznetsova, M. A. Spitsyn, V. E. Shershov, T. O. Guseinov, E. E. Fesenko, S. A. lapa, A. Yu. Ikonnikova, M. A. Avdonina, T. V. Nasedkina, A. S. Zasedatelev, A. V. Chudinov, “Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction”, Mendeleev Commun., 26:2 (2016), 95–98
Образцы ссылок на эту страницу:
https://www.mathnet.ru/rus/mendc2121
https://www.mathnet.ru/rus/mendc/v26/i2/p95
Эта публикация цитируется в следующих 15 статьяx:
Е. V. Suprun, S. A. Khmeleva, K. G. Ptitsyn, L. K. Kurbatov, S. P. Radko, “Artificial modified nucleotides for the electrochemical detection of nucleic acid amplification products”, Zhurnal Analiticheskoi Khimii, 79:7 (2025), 679
E. V. Suprun, S. A. Khmeleva, K. G. Ptitsyn, L. K. Kurbatov, S. P. Radko, “Artificial Modified Nucleotides for the Electrochemical Detection of Nucleic Acid Amplification Products”, J Anal Chem, 79:7 (2024), 831
Svetlana A. Khmeleva, Elena V. Suprun, Yuliana A. Vasileva, Konstantin G. Ptitsyn, Leonid K. Kurbatov, Valeriy E. Shershov, Viktoriya E. Kuznetsova, Alexander V. Chudinov, Sergey P. Radko, Andrey V. Lisitsa, “Effect of poly(dA) stretch in a template sequence on the rate and yield of polymerase chain reaction with electroactive modified 2′-deoxyuridine-5′-triphosphates”, Microchemical Journal, 207 (2024), 111970
Elena V. Suprun, Konstantin V. Bibik, Svetlana A. Khmeleva, Konstantin G. Ptitsyn, Leonid K. Kurbatov, Sergey P. Radko, “Polymerase incorporation of Cy5 modified 2′-deoxyuridine-5′-triphosphate into double- and single-stranded DNA for direct electrochemical detection”, Journal of Electroanalytical Chemistry, 962 (2024), 118240
Hae Min Yang, Jie Ying Teoh, Guk Hee Yim, Yongdoo Park, Young Gyu Kim, Jongseong Kim, Dongwon Yoo, “Label-Free Analysis of Multivalent Protein Binding Using Bioresponsive Nanogels and Surface Plasmon Resonance (SPR)”, ACS Appl. Mater. Interfaces, 12:5 (2020), 5413
V. E. Shershov, A. Yu. Ikonnikova, V. A. Vasiliskov, S. A. Lapa, R. A. Miftakhov, V. E. Kuznetsova, A. V. Chudinov, T. V. Nasedkina, “The Efficiency of DNA Labeling with Near-Infrared Fluorescent Dyes”, BIOPHYSICS, 65:5 (2020), 736
T. O. Guseinov, V. E. Kuznetsova, V. E. Shershov, M. A. Spitsyn, S. A. Lapa, A. S. Zasedatelev, A. V. Chudinov, “New Synthetic Route to CY5-Labeled 2'-Deoxycytidine- 5'-Triphosphates Using Sonogashira Reaction”, Russ J Bioorg Chem, 44:2 (2018), 252
Pedro Güixens-Gallardo, Zbigniew Zawada, Ján Matyašovský, Dmytro Dziuba, Radek Pohl, Tomáš Kraus, Michal Hocek, “Brightly Fluorescent 2′-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry”, Bioconjugate Chem., 29:11 (2018), 3906
V. E. Shershov, S. A. Lapa, V. E. Kuznetsova, M. A. Spitsyn, T. O. Guseinov, S. A. Polyakov, A. A. Stomahin, A. S. Zasedatelev, A. V. Chudinov, “Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip”, J Fluoresc, 27:6 (2017), 2001
М.А. Спицын, В.Е. Кузнецова, В.Е. Шершов, С.А. Лапа, Т.О. Гусейнов, А.С. Заседателев, А.В. Чудинов, “2'-ДЕЗОКСИУРИДИН-5'-ТРИФОСФАТЫ, МОДИФИЦИРОВАННЫЕ ЦИАНИНОВЫМ КРАСИТЕЛЕМ: СИНТЕЗ, ПРИМЕНЕНИЕ И ВЛИЯНИЕ СТРОЕНИЯ ЛИНКЕРА НА СУБСТРАТНЫЕ СВОЙСТВА ДЛЯ Taq-ДНК-ПОЛИМЕРАЗЫ, “Биоорганическая химия””, Биоорганическая химия, 2017, № 4, 444
Igor P. Smirnov, Natalia A. Kolganova, Vadim A. Vasiliskov, Alexander V. Chudinov, Edward N. Timofeev, “Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases”, Sci Rep, 7:1 (2017)
Maksim A. Spitsyn, Viktoriya E. Kuznetsova, Valeriy E. Shershov, Мarina А. Emelyanova, Teimur O. Guseinov, Sergey A. Lapa, Tatyana V. Nasedkina, Alexander S. Zasedatelev, Alexander V. Chudinov, “Synthetic route to novel zwitterionic pentamethine indocyanine fluorophores with various substitutions”, Dyes and Pigments, 147 (2017), 199
A. Yu. Ikonnikova, T. S. Lisitsa, V. E. Shershov, M. A. Spitsyn, T. O. Guseinov, D. O. Fesenko, S. A. Lapa, V. E. Kuznetsova, A. S. Zasedatelev, A. V. Chudinov, T. V. Nasedkina, “The Effect of the Structure of Fluorescently Labeled Nucleotide Derivatives on the Efficiency of Their Incorporation in DNA in the Polymerase Chain Reaction”, BIOPHYSICS, 62:6 (2017), 900
M. A. Spitsyn, V. E. Kuznetsova, V. E. Shershov, S. A. Lapa, T. O. Guseinov, A. S. Zasedatelev, A. V. Chudinov, “Cyanine-dye-modified 2′-deoxyuridine-5′-triphosphates: Synthesis, applications, and linker effect on substrate properties for Taq DNA polymerase”, Russ J Bioorg Chem, 43:4 (2017), 471
V. E. Shershov, V. E. Kuznetsova, S. A. lapa, M. A. Spitsyn, T. O. Guseinov, Ya. V. Tkachev, A. S. Zasedatelev, A. V. Chudinov, “Synthesis and characterization of novel zwitterionic heptamethine indocyanine fluorophores”, Mendeleev Commun., 27:4 (2017), 360–362
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